Hi Modeller experts, Here is my problem. I would like to build a model of a 2 domain protein. I have templates for each domain as well as additional interdomain distance restraints from crosslinking data. As I understand it is possible to introduce these additionnal distance restraints in the input file. However I also notice from FAQ n°1 that in general :"...the relative orientation of the two domains the resulting model will probably have an incorrect relative orientation of the two domains ...". So I suspect there will be a conflict between my (correct) additionnal interdomain distance restraints and the (incorrect) interdomain distance restraints resulting from the initial arbitrary relative orientation of the 2 templates. How can I solve this. Is there a way to tell Modeller not to use these latter restraints or a way to remove these at some point ? Thank you in advance for your help. Best regards.
Michel
Michel Seigneuret Institut Cochin, U567-UMR8104 Département de Biologie Cellulaire 22 rue Méchain, 75014 Paris, France Tel: 33 (0)1 40 51 64 50 Fax: 33 (0)1 40 51 64 54