Thomas Evangelidis a écrit : > Apart from sequence similarity to the target sequence and > crystallographic resolution, someone told me I should also look to the > electron density map info. Yet his english is not good and I haven't > found yet the parameter he's referring to. Can anyone clarify what other > parameters I should look to in order to select the best templates for > modelling? > > thanks, > Tom
I assume he was referring to the 'temperature' factor in the pdb file. Using a lot of viewers, you can color atoms by this "temperature" factor and have a qualitative indication of where your template is "rigid" (low temperature factor), or is more flexible (higher temperature factor).
If you target needs a "hot" section of your template, you'll probably need to pay more attention to the modeling process, since the coordinates from these regions are prone to larger deviations.