Hi,
assume, I have a PDB file template with some residues missing, e.g. 5 Amino acids at the beginning, 10 in the middle and 2 at the end of the sequence.
Now, I use Modeller to complete the template (I align the PDB to its sequence) and use it for homology modelling.
Is this a common procedure? What do you think about it? Will the completed template be reliable or should I leave the gaps of the template unfixed in homology modelling?
Thanks in advance,
Christoph Nimptsch
------------------------------------------ Christoph Nimptsch Apotheker Pharmazeutisches Institut Arbeitskreis Prof. Kovar Universität Tübingen Auf der Morgenstelle 8 D-72076 Tuebingen Tel.: 07071 / 29 73047 Fax.: 07071 / 29 2470 mailto:christoph.nimptsch@uni-tuebingen.de ------------------------------------------