Dear users,
I am getting this error, when I am running modeller (sailing.py): _modeller.ModellerError: fit2xyz_296E> Number of equivalent positions < 3: 0 I am trying to make a multi-template model to reconstruct a loop region for a protein. I am using a experimental structure of the loop (it has only 7 aminoacids), and several whole structures from the PDB and derived from modeller. There is any way to force the alignement or to solve this problem?
Thanks for your attention,
Hocine El Khaoudi Enyoury
On 1/27/22 3:56 AM, Hocine EL KHAOUDI ENYOURY wrote: > I am getting this error, when I am running modeller (sailing.py): > > _modeller.ModellerError: fit2xyz_296E> Number of equivalent positions < 3: 0 > > I am trying to make a multi-template model to reconstruct a loop > region for a protein. I am using a experimental structure of the loop > (it has only 7 aminoacids), and several whole structures from the PDB > and derived from modeller. There is any way to force the alignement or > to solve this problem?
You can't make a structural alignment unless there's at least some structural overlap between the sequences. Sometimes it can help to do a sequence alignment first to get an "initial guess" for the subsequent structural alignment.
Ben Webb, Modeller Caretaker