I am trying to build a protein model.
Is it possible to build the model with the generated PDB files (with lowest DOPE score).
There are 2 regions I which to build, where the first region consist of 1 template & the second region consists of 7 templates.
I have built 100 models each for the 2 regions using single template approach and multiple template approach for the latter.
>From the 100 models, I pick the best model with lowest DOPE score.
With that, I have 1 model for first region & 1 model for second region.
I would like to use the generated 2 models, combine them to build a final model.
To my understanding, I can't proceed straight to the 'model building' command as it doesn't append PDB file.
And I was trying to generate the alignment file for the 2 models but it won't work either.
Could any of you enlighten me on this?
Thank you & have a nice day!
On 9/29/15 11:33 PM, Wong Li Zhe wrote: > I would like to use the generated 2 models, combine them to build a > final model.
In this case your alignment would contain all 8 templates, 7 for one region and 1 for the other. If you really want to do this in two steps you can take the 2 models and use them as "templates" for modeling as in the FAQ, but it'll be less efficient.
Ben Webb, Modeller Caretaker