On 8/31/23 11:25 PM, Arun Dhanasekaran via modeller_usage wrote: > My reference pdb contains two chains of different protein, one with 306 > residues in which 39 residues are missing and another with 271 residues > with 22 residues are missing
As others have said, adding missing residues for a multi-chain protein is exactly the same as for a single-chain protein, except that you need to add chain break characters ("/") between the chain sequences. Alternatively, add the missing residues for each chain individually and then combine the two models into one, e.g. with a package such as ChimeraX.
Ben Webb, Modeller Caretaker