Dear Sir: I would like to build models assining different templates for different regions. For exmple: PDB-A for 1-150aa, PDB-B for 151-220aa and so on. How to achieve this? Because different template has different homolog with different regions. Thank you very much.
Does anybody know how to achieve this? Thank you very much.
在2009-11-22,leuven leuven@yeah.net 写道: -----原始邮件----- 发件人:leuven leuven@yeah.net 发送时间:2009年11月22日 星期日 收件人:modeller_usage@salilab.org 主题:[modeller_usage] how to assign different template for different regions?
Dear Sir: I would like to build models assining different templates for different regions. For exmple: PDB-A for 1-150aa, PDB-B for 151-220aa and so on. How to achieve this? Because different template has different homolog with different regions. Thank you very much.
Hello,
First have a look at how you build a model from multiple templates: http://salilab.org/modeller/manual/node21.html
Then just make sure that, in your alignment, the templates align with the part of the protein you want to model.
>P1; PDBA structureX:PDBA: 1: A: 10: A:::: AAAAAAAAA--------------------* >P1; PDBB structureX:PDBB: 1: A: 10: A:::: ---------------------BBBBBBBBB* >P1; Model sequence:Model: 1: A: 20: A:::: AAAAAAAAABBBBBBBBB*
I *think* this is the correct approach but honestly I can't be certain because I can't check it now.
Regards,
João [...] Rodrigues @ http://stanford.edu/~joaor/
2009/11/24 leuven leuven@yeah.net
> > Does anybody know how to achieve this? > Thank you very much. > > 在2009-11-22,leuven leuven@yeah.net 写道: > > -----原始邮件----- > *发件人:*leuven leuven@yeah.net > *发送时间:*2009年11月22日 星期日 > *收件人:*modeller_usage@salilab.org > *主题:*[modeller_usage] how to assign different template for different > regions? > > > Dear Sir: > I would like to build models assining different templates for different > regions. For exmple: PDB-A for 1-150aa, PDB-B for 151-220aa and so on. How > to achieve this? Because different template has different homolog with > different regions. > Thank you very much. > > > _______________________________________________ > modeller_usage mailing list > modeller_usage@salilab.org > https://salilab.org/mailman/listinfo/modeller_usage > >